期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1992
卷号：13
期号：3
页码：169-178
DOI：10.3164/jcbn.13.169
出版社：The Society for Free Radical Research Japan
摘要：The purpose of these experiments was to determine whether resident murine peritoneal macrophages possess desaturase activity to convert stearic acid into oleic acid. Peritoneal macrophages were purified by adherence and incubated in a medium containing fatty acid-free BSA with [1-14C] stearic acid (18:0). Incorporation of [1-14C] 18:0 into macrophage lipids increased up to 4h (>55%) after incubation and then declined in a linear fashion over the next 20h. Majority of the [1-14C] 18:0 incorporated into macrophages remained as free acid throughout the incubation period. Incorporation of [1-14C] 18:0 and desaturated products into the phospholipid fraction increased linearly up to 16h and then decreased at 24h of incubation. A small amount of radioactivity was also associated with triacylglycerol and cholesterol ester fractions. The relative incorporation into phospholipid classes reached a plateau at 4h and remained unchanged throughout the 24-h incubation period. Resident macrophages possessed the capability to desaturase [1-14C] 18:0 into oleic acid, and this rate of desaturation was maximum at 8h. These results clearly demonstrate that murine peritoneal macrophages have the ability to synthesize oleic acid and to incorporate it into various lipid fractions.
