期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1993
卷号：15
期号：3
页码：155-162
DOI：10.3164/jcbn.15.155
出版社：The Society for Free Radical Research Japan
摘要：8-Hydroxydeoxyguanosine (8-OH-dG) was formed in deoxyguanosine (dG) solution by the addition of Fe2+, ascorbic acid (AscA), EDTA, and H2O2, and was then detected by ECD after separation by HPLC. 8-OH-dG was not formed in significant amounts when either Fe2+, AscA, or H2O2 was added separately, or when two of these were added in combination. When Fe2+, AscA, and H2O2 were added simultaneously, however, 0.87nmol of 8-OH-dG was formed from 2.5μmol of dG. And the addition of EDTA (in the same concentration as Fe2+) to the Fe2+/AscA/H2O2 system caused a remarkable increase in the amount of 8-OH-dG formed (up to 2.89nmol from 2.5μmol of dG). The amount of 8-OH-dG formed increased as the concentration of AscA increased up to 1mM (the same concentration as Fe2+). However, higher concentrations of AscA inhibited the formation of 8-OH-dG.
