期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1994
卷号：16
期号：1
页码：51-58
DOI：10.3164/jcbn.16.51
出版社：The Society for Free Radical Research Japan
摘要：Metabolic changes in folic acid and their polyglutamyl derivatives were studied in streptozotocin-induced diabetic rats. Polyglutamylfolates, which constituted about 70% of the folates (determined by the Lactobacillus casei microbiological assay) in normal rat liver, were observed to be hydrolyzed to a greater extent to simple monoglutamyl forms as the diabetes progressed. Only 4-5% conjugated folates were observed 25 days after the injection of streptozotocin. Total methylfolates, which represented 70-75% of total folates, were lowered by 60% and methylpolyglutamylfolates by 95% in the diabetic rat. An imbalance in the two folate-metabolizing enzymes, folyl conjugase and folylpolyglutamate synthetase, were observed in diabetes, the former being 2-3-fold higher and the latter being 7-8-fold lower. [14C]Folate uptake studies indicated that folates accumulated for up to the first 24h in diabetic rat liver, and then were depleted over the next 48h period, indicating an impaired polyglutamylation and storage of folates.
