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  • 标题:Partial Purification of Immunoreactive Basic Fibroblast Growth Factors from Sera of Patients with Breast Cancer and Investigation of Their Mitogenic Activity toward BALB/c3T3 Fibroblasts
  • 本地全文:下载
  • 作者:Yoshifumi TAKEI ; Hanae HIGASHIRA ; Kyozo HAYASHI
  • 期刊名称:Journal of Clinical Biochemistry and Nutrition
  • 印刷版ISSN:0912-0009
  • 电子版ISSN:1880-5086
  • 出版年度:1994
  • 卷号:17
  • 期号:1
  • 页码:19-28
  • DOI:10.3164/jcbn.17.19
  • 出版社:The Society for Free Radical Research Japan
  • 摘要:In a previous study, we detected in human serum two immunoreactive bFGF-like (bFGF-LI) substances, a high-molecular-weight form (designated as HMW-bFGF-LI) and a 16-kDa form. In this current work, we partially purified these two Immunoreactive bFGFs from sera of patients with breast cancer by heparin-affinity chromatography and gel filtration, and further investigated their immunochemical properties and biological activities. As a result, we found that the antigenicity of the two immunoreactive bFGFs was identical to that of recombinant bFGF. And the mitogenic activity toward BALB/c3T3 fibroblasts of the 16-kDa bFGF-LI was equal to that of recombinant bFGF, whereas the mitogenic effect of HMW-bFGF-LI was weak. The mitogenic activity of the two Immunoreactive bFGFs was completely inhibited by the addition of neutralizing antibody. The mitogenic activity of the 16-kDa bFGF-LI was increased by the addition of dexamethasone, whereas that of HMW-bFGF-LI was unaffected by the drug.
  • 关键词:basic fibroblast growth factor (bFGF);breast cancer;mitogenic activity;high-molecular-weight bFGF-like immunoreactive substance;BALB/c3T3 fibroblasts
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