标题：Protective Effect of Catecholestrogen on Lipid Hydroperoxide-Induced Injury to Cultured Bovine Aortic Endothelial Cells and on Lipid Peroxidation of Low-Density Lipoprotein
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1995
卷号：19
期号：2
页码：71-77
DOI：10.3164/jcbn.19.71
出版社：The Society for Free Radical Research Japan
摘要：When bovine aortic endothelial cells were incubated in Dulbecco's modified Eagle's medium containing 5% fetal bovine serum and 5nmol/ml (in terms of malondialdehyde) of linoleic acid hydroperoxide, release of lactate dehydrogenase (LDH) from the cells into the medium increased over a 2-4h incubation period, reaching a maximum level of about 80% of the total activity of LDH. In the presence of 17β-estradiol (E2) or 2-hydroxyestradiol (2-OHE2), the LDH release was inhibited depending on their concentration. The IC50 values of the inhibition were approximately 5 and 1μM, respectively. The lipid peroxide level in the cultured endothelial cells increased upon incubation with the hydroperoxide, which increase was suppressed markedly by 2-OHE2 and slightly by E2. Lipid peroxidation of low-density lipoprotein was inhibited markedly by 2-OHE2 and slightly by E2.
