期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1995
卷号：19
期号：2
页码：89-95
DOI：10.3164/jcbn.19.89
出版社：The Society for Free Radical Research Japan
摘要：In order to investigate mucin production in various pathological states, we generated murine monoclonal antibodies (4H6, 2D11) against mucins from bronchio-alveolar lavage fluids (BALE) of hamsters with bronchitis caused by sulfur dioxide exposure. In the immunohistochemical studies, the antibodies recognized the mucins secreted into the tracheal lumen, but not the mucins stored in goblet cells or in submucosal gland mucous cells. ELISA showed that the antibodies react with mucins from hamster intestine and swine stomach, and with BALF of rats, guinea pigs, dogs, and humans. However, the antibodies recognized neither bovine submaxillary gland mucins nor proteoglycans. In conclusion, the ELISA using the monoclonal antibodies is available to quantify airway mucin production.
