标题：Endocytosis of Myeloperoxidase by Human Monocyte-Derived Macrophages and Multistep Regulation of Mannose Receptor Activity during Macrophage Differentiation
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1998
卷号：25
期号：3
页码：109-119
DOI：10.3164/jcbn.25.109
出版社：The Society for Free Radical Research Japan
摘要：Myeloperoxidase (MPO) purified from human neutrophils was endocytosed by human monocyte-derived macrophages with a K of uptake of 10.6nM and a K d of 27.8nM. Fucoidan and mannan inhibited the uptake of MPO into the macrophages, indicating that the uptake was mediated by mannose/fucose receptors. Internalized MPO was degraded with a half time of 5.5h, and the degradation was inhibited by chloroquin. The presence of cytokines during the differentiation of monocytes into macrophages caused enhancement of the endocytosis of MPO by macrophage-colony stimulating factor (M-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon-γ (IFN-γ), interleukin-5 (IL-5), and transforming growth factor-β (TGF-β), and inhibition of it by interferon-α (IFN-α). The stimulatory effect of IFN-γ or GM-CSF was antagonized by IFN-α, but that of TGF-β was not. In differentiated macrophages, the endocytosis was stimulated by IFN-α and TGF-β, while it was inhibited by IFN-γ. Expression of the receptor seems to be under multistep control during macrophage differentiation.
