期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1999
卷号：26
期号：1
页码：35-50
DOI：10.3164/jcbn.26.35
出版社：The Society for Free Radical Research Japan
摘要：To assess the appropriate content of vitamin E (VE) in fish oil capsules, we determined lipid peroxide and VE levels in human blood after hyperlipidemic female volunteers had heed given purified eicosapentaenoic acid (EPA) ethyl ester (90% purity) in capsules along with a controlled basal diet. A two consecutive 7-day menu cycle was used, and the capsules were taken with the second 7-day menu. The basal diet was based on the recommended dietary allowances for female Japanese with light physical activities. The fat energy density of the diet was 25%, and the cholesterol intake was less than 300mg per day. Each volunteer ingested nine capsules (2.43g of EPA ethyl ester in total) daily in three divided doses, and a daily total of 5.4mg of all- rac -α-tocopherol (4mg RRR -α-tocopherol equivalent) from the capsules. Blood was collected before and after 1 and 2 weeks of the basal diet periods. Triacylglycerol and total cholesterol concentrations in plasma were determined, and plasma lipid peroxide levels as estimated by thiobarbituric acid reactive substances (TBARS) and water-soluble fluorescent substances (WSF) were also measured. In addition, α-tocopherol levels in plasma, red blood cells (RBC), and platelets were analyzed. The plasma total cholesterol concentration decreased significantly after supplementation with the capsules, but that of triacylglycerol did not change significantly. The TBARS values expressed as plasma concentration and in terms of plasma lipid levels did not change significantly in each case. An amelioration of plasma lipid levels effectively lowered the plasma concentration of WSF. The plasma α-tocopherol concentration did not change significantly with the treatment with the capsules, although the concentration decreased significantly on the controlled basal diet with lower energy but without the supplementation. Neither the α-tocopherol content in RBC nor that in platelets changed significantly during the test period. In conclusion, the VE content in the EPA capsules appears to be sufficient to provide adequate antioxidant protection during the short period of the experiment. An assessment of appropriate VE content in fish oil capsules is discussed by comparing the current data with those reported elsewhere.
