期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2000
卷号：28
期号：2
页码：53-58
DOI：10.3164/jcbn.28.53
出版社：The Society for Free Radical Research Japan
摘要：Two Shiga toxin (Stx) genes ( stx -1 and stx -2) were cloned and sequenced from a strain of enterohemorrhagic Escherichia coli , serotype O157:H7, a clinical isolate from the Gifu City, Japan, outbreak in 1996 (GPU96MM). The 1, 257 by of stx -1 and 1, 321 by of stx -2 genes were analyzed, and each gene contained two open reading frames (ORFs), 948 or 960 by of A and 270 by of B subunits, respectively. Although the nucleotide sequences of stx -1 ORFs of this isolate had several nucleotide alterations, the deduced amino acid sequence was identical to that of stx -1 from E. coli (O157:H7) isolated from Canada and stx from Shigella dysenteriae type 1. The nucleotide sequences of the stx -2 ORFs of this strain were identical to those of stx -2 from two E. coli (O157:H7) strains, KNIH317 isolated from Korea and 933.
