期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1987
卷号：3
期号：1
页码：27-36
DOI：10.3164/jcbn.3.27
出版社：The Society for Free Radical Research Japan
摘要：High density lipoprotein 3 (HDL3=1.12< d <1.21) was modified with dilinoleoylphosphatidylcholine, and the effects of these modified HDL3 on the removal of [3H]cholesterol from rat peritoneal macrophages were studied. On incubation of HDL3 with polyenephosphatidylcholine (PPC) vesicles, phospholipid-rich high density lipoproteins (1.18< d <1.24) were formed. The apolipoprotein AI (apo AI) and AII (apo AII) contents of these modified HDL3 were about 3 to 40% of those of native HDL3. Removal of [3H]cholesterol from macrophages was investigated using rat peritoneal macrophages prelabeled with [3H]cholesterol emulsion or with [3H]cholesterol oleate-labeled acetylated low density lipoproteins (1.019< d <1.063). The modified HDL3s facilitated the removal of [3H]cholesterol from macrophages. The extent of removal of [3H]cholesterol from macrophages was higher with modified HDL3 than with native HDL3, and among modified HDL3 subfractions, fractions IV and V, whose ratio of apo AI to phospholipid (AI/PL) were 0.44 and 0.58, respectively, were more efficient [3H]cholesterol acceptors than native HDL3 (AI/PL=1.46). These results indicate that the effectiveness of HDL3 in removing [3H]cholesterol from macrophages was enhanced by modification with PPC.
关键词：dilinoleoylphosphatidylcholine;high density lipoprotein;macrophages;apo AI;cholesterol release
