期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1987
卷号：3
期号：2
页码：159-168
DOI：10.3164/jcbn.3.159
出版社：The Society for Free Radical Research Japan
摘要：The diurnal change in endogenous plasma triglyceride lipase (TGL) activity has not yet been studied in detail together with lipid-apoprotein profiles in man. Therefore, this study was conducted to observe the fluctuations from morning to night in the TGL activity including lipoprotein lipase (LPL) and hepatic lipase (HL) activities in 8 healthy subjects, first on a high-carbohydrate diet (carbohydrate, 360g; protein, 60g; fat, 20g) and then on an isocaloric high-fat diet (carbohydrate, 180g; protein, 60g; fat, 100g). Each diet was divided into 3 similar portions and given at 8:00, 12:00, and 18:00h. Blood sampling was done every 2h. Plasma glucose showed no definite change during the day of each test diet, while plasma insulin levels were much higher with the high-carbohydrate diet than with the high-fat one. Plasma triglyceride (TG) in chylomicron (Chyl) and very low density lipoprotein (VLDL) revealed a significantly greater increment with the high fat diet than with the high-carbohydrate diet. Levels of plasma apoproteins C-II, C-III, and E increased only with the high-fat diet. The diurnal endogenous plasma TGL activity as measured by a sensitive method was raised with both diets in parallel with the Chyl-VLDL-TG levels, and the elevated TGL activity was mostly due to the accelerated release of HL, and not to that of LPL. Unexpectedly no correlation existed between diurnal plasma TGL activity and insulin secretion. Interestingly, however, a remarkable positive correlation ( r =0.744, p <0.01) was found between the diurnal sum of plasma TGL activity changes and the diurnal sum of plasma Chyl-VLDL-TG changes. Thus, the diurnal TG increment in the carrier lipoproteins induced by meals seems to accelerate the release of TGL, and therefore HL, from the hepatic capillaries into circulation, independent of insulin secretion.
