期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2001
卷号：30
页码：67-77
DOI：10.3164/jcbn.30.67
出版社：The Society for Free Radical Research Japan
摘要：Highly sensitive and rapid assay procedures for human mitochondrial the pyruvate dehydrogenase (PDH) complex, the 2-oxoglutarate dehydrogenase (OGDH) complex and their 5 component enzymes were established for use with crude tissue extracts, and their enzymatic activities were determined. Overall reaction activities of the PDH and OGDH complexes were very high, in order of heart>abdominal skeletal muscle>kidney. Full-length cDNA encoding the precursor of theporcine dihydrolipoamide acetyltransferase (E2p) subunit was isolated and characterized. It contained an open reading frame encoding a leader peptide (86 residues) and a mature enzyme protein (561 residues, M r=59, 699). The reduced enzyme protein showed 91% identity with the human enzyme. All genes encoding the component enzymes of the 2 human complexes were expressed in a variety of organs, but most strongly in heart and skeletal muscle (abdominal muscle) in a similar order as found for their enzymatic activities.
关键词：human PDH and OGDH complexes;5 component enzymes;enzymatical assay;cloning of porcine E2p cDNA;Northern blot analysis
