期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1988
卷号：5
期号：1
页码：51-57
DOI：10.3164/jcbn.5.51
出版社：The Society for Free Radical Research Japan
摘要：Some properties of epidermal growth factor (EGF)-like immunoreactive substances (EGF-LI) found in human platelets were studied. Human platelets were treated with acidic, alkaline, or hypotonic solutions or by freezing-thawing. Then after centrifugation the hEGF-LI in the resulting supernatant was examined by our rapid enzyme immunoassay system for the presence of hEGF-LI's released from the platelets. The amount of hEGF-LI released from platelets varied with the treatment, the alkaline or freezing-thawing treatment being the most effective in liberating the hEGF-LI. The hEGF-LI was applied to a column of Sephadex G-100 and was detected in four fractions showing molecular weights of about 280, 56, 20, and 6kdaltons. The high molecular weight hEGF-LI's having molecular weights of 280 and 56kD were converted to 20kD one by treatment with 2-mercaptoethanol. So it appears that some of the hEGF-LI's in platelets may exist in a complex form through disulfide linkages with some proteins in the platelets.
关键词：epidermal growth factor;human platelets;liberation;biochemical properties;complex form
