期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1988
卷号：5
期号：2
页码：127-134
DOI：10.3164/jcbn.5.127
出版社：The Society for Free Radical Research Japan
摘要：Fe2+ and Fe3+ bound to protein in human serum were released by addition of TCA-HCl mixture after elimination of coexisting ascorbic acid, uric acid, etc. by centrifugation with an ultrafiltration membrane cone filter or after decomposition of these reducing agents with ascorbate oxidase and uricase. Fe2+ in the supernatant of the TCA-HCl mixture was determined at 535nm by the bathophenanthroline method, and the Fe3+ in the supernatant was reduced to Fe2+ by addition of ascorbic acid and successively assayed as Fe2+ by the bathophenanthroline method. Changes in the amount of Fe2+ and Fe3+ bound to human serum protein over a 24-h period were then studied by using the present method. A peak of Fe3+ in human serum seemed to appear in the morning, while almost no peak was observed for Fe2+ (average concentration: 10μM). So, the ratio of Fe2+/Fe3+ bound to human serum protein varied over the range of 0.5-5.0 within a day. The normal range of total iron concentration in human serum was 12-28μM.
关键词：Fe2+ in serum;Fe3+ in serum;successive colorimetry of Fe2+ and Fe3+ in serum;bathophenanthroline;change in serum Fe2+/Fe3+
