期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1988
卷号：5
期号：3
页码：241-254
DOI：10.3164/jcbn.5.241
出版社：The Society for Free Radical Research Japan
摘要：This study evaluates the effect of continuous fluid infusion on metabolic alterations in septic rats. The saline administration, designed to maintain blood volume, was carried out from the onset of peritonitis induced by cecal ligation and puncture. The operated rats were divided into two groups: the physiological saline-resuscitated group, treated by continuous infusion intravenously (2.0ml/h) immediately after surgery, and the untreated septic group. Concentrations of hepatic adenine nucleotides, glycogen, glucose-6-phosphate, phosphoenolpyruvate, lactate, malate, RNA, DNA, and protein, were determined at the times of 4, 7, 12, and 24h. Alterations in free amino acid levels in plasma and liver were also measured. A rapid decrease in plasma glucogenic amino acids and perturbation of carbohydrate metabolism were noted at 4h and alterations in metabolite levels became evident at 7h in both septic rat groups, when compared with those in normal rats. In addition, obvious differences in the alteration patterns of metabolite contents were observed at 12h between the two septic rat groups. The untreated septic rats appeared to be in a shock state at 12h, showing approximately 50% mortality, whereas all septic rats with resuscitation still survived at this time. These results indicate that an adverse effect of lowered tissue perfusion was involved in further aggravation of hepatic ATP-producing metabolism in the untreated septic rats.
关键词：fluid resuscitation;metabolite levels;septic rats;carbohydrate and protein metabolism
