期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1989
卷号：6
期号：2
页码：147-153
DOI：10.3164/jcbn.6.147
出版社：The Society for Free Radical Research Japan
摘要：A chemotactic factor for polymorphonuclear leukocytes was found in the culture fluid of human lung giant cell carcinoma cell line Lu65. This factor was defined as chemotactic one by checkerboard analysis. Partial purification of the chemotactic factor was performed by DEAE-Sepharose and gel filtration with Sephadex G-100, which showed it to be basic and low molecular weight. The factor was also found in the filtrate of culture fluid passed through a Centriflo CF25 filter, thus indicating a molecular weight of less than 25kDa. Furthermore, three cell clones having higher activity of chemoattractant were selected from the original cell line.
