期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1989
卷号：6
期号：3
页码：195-204
DOI：10.3164/jcbn.6.195
出版社：The Society for Free Radical Research Japan
摘要：Effect of dietary riboflavin deficiency on rat lens glutathione, glutathione redox cycle, and oxidative-antioxidative processes was investigated to understand the possible biochemical mechanism(s) responsible for aggregation of lens proteins. Induction of riboflavin deficiency resulted in a marked reduction in the activity of glutathione reductase (GSH-R). Levels of glutathione (both reduced and oxidised) and sulfhydryl groups (total and non-protein), however, were unchanged. Activity of glucose 6-phosphate dehydrogenase, which generates the reducing equivalents, NADPH, necessary for maintenance of normal GSH-R activity, was found to be unaltered. On the other hand, glutathione dependent glutathione peroxidase, a potent peroxide scavenging enzyme, showed a significant rise in its activity with a concomitant increase in lipid peroxidation. Among the other antioxidative systems studied, superoxide dismutase and catalase remained unaltered, while levels of ascorbate declined. These results indicate impairments in the glutathione redox cycle and antioxidative defence mechanisms, in addition to enhanced lipid peroxidation in the lens of riboflavin deficient rats.
关键词：rat lens;riboflavin deficiency;oxidant-antioxidant system;aggregation of proteins
