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  • 标题:Identification of Cobalamin Binding Protein Synthesized de novo by Gastric Cancer Cells
  • 本地全文:下载
  • 作者:Yoshio WAKATSUKI ; Masami INADA ; Hiroyuki KUDO
  • 期刊名称:Journal of Clinical Biochemistry and Nutrition
  • 印刷版ISSN:0912-0009
  • 电子版ISSN:1880-5086
  • 出版年度:1989
  • 卷号:7
  • 期号:1
  • 页码:43-51
  • DOI:10.3164/jcbn.7.43
  • 出版社:The Society for Free Radical Research Japan
  • 摘要:The content of cobalamin-binding protein (Cb1BP) was measured in stomach extracts and various cell lines. Cb1BP was increased in the cancer extract compared with non-cancerous control. The level of Cb1BP was also increased in serum-free culture medium of a gastric cancer cell line, but was almost undetectable in media of control cell lines. This output of Cb1BP in medium was suppressed by cycloheximide. These Cb1BPs were immunologically cross-reactive with anti saliva R binder antibody. The M r, determined by immunoprecipitation, was 70kDa and the pI, 2.8-3.2. From these properties, this Cb1BP in cancer cells was considered to be an isoprotein of the cobalamin R binder. Histochemical studies showed that immunoreactive Cb1BP was confined to cytosol of cancer cells and cells in metaplasia. The present results suggest that Cb1BP of the R binder type is synthesized de novo in some type of gastric cancer cells and in metaplastic cells.
  • 关键词:cobalamin binding protein;serum-free culture;immunoprecipitation;immunohistochemistry;R binder
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