期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1989
卷号：7
期号：3
页码：185-192
DOI：10.3164/jcbn.7.185
出版社：The Society for Free Radical Research Japan
摘要：A pCH 110 plasmid encoding the β-galactosidase gene of E. coli was effectively entrapped in novel positively charged liposomes consisting of N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine (1:2:2, molar ratio) by an improved reverse-phase evaporation method. The DNA entrapping efficiency of these liposomes was 5-70 fold higher than that of liposomes containing no positively charged lipid. By using such positively charged liposomes, we could achieve transfection of the gene into COS-1 cells by simply adding the liposomes to the medium covering the cells; and the transfection efficiency exceeded that obtained by the calcium phosphate precipitation method, especially when a small amount of DNA was used.
