期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1990
卷号：8
期号：2
页码：111-119
DOI：10.3164/jcbn.8.111
出版社：The Society for Free Radical Research Japan
摘要：Antitumor effect of sulfatide-inserted liposomes (composed of egg phosphatidylcholine, cholesterol, and sulfatide in a molar ratio of 5:4:1) containing entrapped adriamycin (ADM) against three types of human gastric cancers implanted in nude mice was examined by the subrenal capsule assay as an in vivo sensitivity test for antitumor agents. The results indicate that the antitumor effect of ADM-containing liposomes was equal or superior to that of free ADM in every tumor tested. To evaluate the clinical usefulness of these liposomes as drug carriers, we measured concentrations of ADM in tissues including tumors after the administration of the liposomes containing ADM, and found that the level in the heart was lower than in the case of free ADM. We also observed that the blood level remained high compared with the case of free ADM. The ADM concentration in the tumor tissue was initially lower, but became higher by 30-40h after the administration, than that in the tumors of animals injected with free ADM. The acute toxicity of ADM entrapped in liposomes as judged by the 50% lethal dose was less than that of free ADM. The body weight loss was also less with ADM-liposomes than with free ADM. Thus, we conclude that entrapment of ADM in sulfatide-inserted liposomes preserves the antitumor activity of ADM and reduces the side effects of the drug.
关键词：sulfatide-inserted liposomes;adriamycin;human gastric cancer
