标题：Measurement by MCLA-Dependent Luminescence Method of Superxide Formation by N-Formylmethionyl-Leucyl-Phenylalanine-Actuate Inflammatory Cells In Vitro
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1990
卷号：8
期号：3
页码：217-225
DOI：10.3164/jcbn.8.217
出版社：The Society for Free Radical Research Japan
摘要：Activities of human neutrophils and eosinophils to generate O2- were investigated by use of 2-methyl-6-[ p -methoxyphenyl]-3, 7-dihydroimidazo [1, 2-α] pyrazin-3-one (MCLA) as a chemiluminescence probe and N-formylmethionyl-leucyl-phenylalanine (fMLP) and cytochalasin B. In some cases, rat mast cells were used instead of human neutrophils and eosinophils. The luminescence intensity was dependent upon the number of neutrophils, and the maximal luminescence intensity was given at 1×10-6M fMLP with a fixed number of the cells. Luminescence intensity caused by the addition of 0.5μM MCLA and 1×10-6M fMLP to a suspension of eosinophils reached maximum at 30s and decreased rapidly thereafter. Biphasic luminescence intensity was observed with 1×10-6M fMLP-stimulated neutrophils and eosinophils in the presence of 5μg/ml cytochalasin B. These MCLA-dependent luminescences by fMLP-stimulated eosinophils were completely abolished by 0.5μM SOD. On the other hand, addition of MCLA and fMLP to a suspension of rat mast cells showed no significant luminescence irrespective of the presence of cytochalasin B.
