标题：Production of Monoclonal Antibodies against Rat Anionic Trypsinogen, and Their Use in Enzyme-Linked-Immunosorbent-Assay Specific for Anionic Trypsinogen
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1990
卷号：9
期号：1
页码：25-37
DOI：10.3164/jcbn.9.25
出版社：The Society for Free Radical Research Japan
摘要：By use of a hybridization technique between BALB/c myeloma cells and spleen cells of mice immunized with rat anionic trypsinogen purified from rat pancreas, four monoclonal antibodies against this protein were produced. These monoclonal antibodies exhibited different inhibitory effects to anionic trypsinogen enzyme assay with benzoyl-L-arginine- p -nitroanilide hydrochloride as a substrate. Several sandwich enzyme-linked-immunosorbent-assays (ELISA's) were produced with different combinations of two monoclonal antibodies. One ELISA showed almost the same reactivity between anionic trypsinogen and anionic trypsin inactivated with an active site-specific reagent. Another ELISA detected anionic trypsinogen specifically, and the cross-reactivity of rat α1-protease inhibitor-bound anionic trypsin and inactivated anionic trypsin were 1.6% and about 4%, respectively. Both ELISA's showed less than 1% cross-reactivity with rat cationic trypsinogen purified from the pancreas.
