标题：Growth Inhibition of Glioma Cells of Different Cell Lines by Human Interferon-β Produced in the Cells Transfected with Its Gene by Means of Liposomes
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：1990
卷号：9
期号：2
页码：73-77
DOI：10.3164/jcbn.9.73
出版社：The Society for Free Radical Research Japan
摘要：Using five different glioma cell lines, SK-MG-1, SK-MG-4, U251-MG, U251-SP, and T98, and one normal glial cell line, we performed transfection of the cells with a human interferon-β (HuIFN-β) gene by means of our novel liposomes. As a result, HuIFN-β was found in the media of all glioma cells, but not in that of normal filial cells. The level of HuIFN-β increased with incubation time until 4 days after the addition of the liposomes, and approximately 20-40IU/ml of HuIFN-β was found in the media of all glioma cells except for U251-SP cells, which produced approximately 350IU/ml of HuIFN-β. Then, the levels decreased gradually and HuIFN-β in the medium could not be detected after 9 days of cultivation. By liposome-mediated transfection of the HuIFN-β gene, remarkable growth-inhibitory effects were observed for all glioma cell lines. At 4 days of incubation the effect in all glioma cell lines exceeded that obtained with 1, 000IU/ml of exogenously added HuIFN-β.
