出版社：American Society for Biochemistry and Molecular Biology
摘要：The clearing factor lipase (lipoprotein lipase) activities of homogenates of fresh tissue and of acetone-ether powders have been compared in hearts from fed and starved rats. The activity of the enzyme measured in homogenates of acetone-ether powders is generally higher than that in homogenates of the fresh tissue. Activation is due to an effect of the acetone-ether treatment on enzyme which is associated with the tissue residue in fresh tissue homogenates. A similar activation occurs when the tissue residue is treated with deoxycholate. When rats are fasted, a marked increase in the clearing factor lipase activity of the heart occurs. Peak activities are reached after 10-24 hr, and thereafter the activity falls slowly. This pattern of activity is observed in homogenates of fresh tissue and of acetone-ether powders. The activity of clearing factor lipase in diaphragm muscle also increases in rats starved for 8 or 24 hr. The importance of the change in muscle clearing factor lipase activity on fasting in relation to triglyceride fatty acid utilization by this tissue is emphasized.
