出版社：American Society for Biochemistry and Molecular Biology
摘要：The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained. ( a ) Plasma lipoproteins were eluted successively from the column in the four overlapping peaks of chylomicrons, very low density lipoproteins, low density lipoproteins, and high density lipoproteins. ( b ) Very low density lipoproteins and high density lipoproteins (d > 1.063, containing nonlipoprotein proteins) showed continuous progressive changes in lipid composition as these fractions emerged, while low density lipoproteins showed a relatively constant lipid composition. ( c ) A discontinuous transition of lipid composition was observed when consecutive ultracentrifugal fractions were placed on the column. ( d ) The ``trail'' of pre-beta lipoprotein seen on paper electrophoresis was shown to consist of particles whose molecular sizes range between chylomicrons and pre-beta lipoproteins. A reverse relationship was observed between electrophoretic mobilities of ``trail'' components and their particle size. ( e ) Gel with an agarose content of 2% seemed to fractionate chylomicrons and very low density lipoproteins more effectively than other lipoprotein classes.
关键词：agarose gel ; hyperlipidemia ; thin-layer chromatography ; paper electrophoresis ; ultracentrifugation
