出版社：American Society for Biochemistry and Molecular Biology
摘要：The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of ( a ) acetylation of total lipids with pyridine and acetic anhydride, ( b ) separation of acetylated glycolipids from nonglycolipids on a magnesia–silica gel (Florisil) column, and ( c ) deacetylation of glycolipid in chloroform–methanol–sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.
