出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple yet powerful new chromatographic procedure for vitamin D3 and its metabolites is described. Liquid–gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl3 in Skellysolve B (petroleum ether, bp 67–69°C) permits excellent resolution of vitamin D3, 25-hydroxyvitamin D3, and their more polar metabolites. Of special importance is the resolution of the metabolites of vitamin D3 more polar than 25-hydroxycholecalciferol. Because of this resolution, a new metabolite of vitamin D3 has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitamin D3-1,2-3H.
