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  • 标题:A simple lipase assay using trichloroacetic acid
  • 本地全文:下载
  • 作者:Michael C. Schotz ; Arlene S. Garfinkel
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1972
  • 卷号:13
  • 期号:6
  • 页码:824-826
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-3H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of 3H-labeled glycerol.
  • 关键词:adipose tissue ; postheparin plasma ; triolein ; 3H-labeled glycerol ; lipoprotein lipase ; triglyceride
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