出版社:American Society for Biochemistry and Molecular Biology
摘要:The profiles of total lipids and of the molecular species of individual lipid classes were compared among corresponding lipoproteins of plasma and yolk of the laying hen. A close qualitative correspondence was found in the makeup of the molecular species of glycerophospholipids and triglycerides of the very low density lipoproteins and the high density lipoproteins of plasma and yolk. There was a lower proportion of the trienoic triglycerides and of the dienoic glycerophospholipids in the egg yolk than in the plasma lipoproteins, and the greatest differences (20–30%) were noted between the high density lipoproteins. It was also observed that the plasma high density lipoproteins lost their cholesteryl esters upon entering the yolk. On the basis of these and comparable analyses of the plasma lipoproteins of the nonlaying hen, it is concluded that the laying hen synthesizes specific lipoproteins for deposition in the yolk, and these are carried in plasma and selectively transferred to the developing ovum without significant equilibration with the other plasma lipoproteins.
关键词:total lipid profiles ; direct gas–liquid chromatography ; phospholipase C digestion ; cholesteryl esters ; molecular species ; triglycerides ; glycerophospholipids