出版社：American Society for Biochemistry and Molecular Biology
摘要：Bile was collected for 18–24 days from adult male rats with cannulated bile ducts that had received intraperitoneally 0.8 mg of 5α-[4-14C, 3α-3H]cholestan-3β-ol. Bile from the first 2 days containing 14.2% of the administered 14C and 3.3% of the 3H was hydrolyzed, and the bile acids were separated by acetic acid partition chromatography. The previously unidentified metabolite more polar than cholic and allocholic acids was identified by isotopic dilution as 3β,7α,l2α-trihydroxy-5a-cholanic acid and represented 3% of the biliary 14C and 15% of the 3H. Similarly, 3β,7α-dihydroxy-5α-cholanic acid was identified in fractions more polar than allochenodeoxycholic acid and represented 0.6% of the biliary 14C and 8% of the 3H. More polar fractions contained 4% of the 14C and 31% of the 3H in unidentified metabolites.
