出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple, rapid method for the determination of cholesterol in plasma and tissue using o -phthalaldehyde is presented. Comparison of this method with the FeCl3 method gave identical results. However, the o -phthalaldehyde determination is three times more sensitive than the FeCl3 determination (molar extinction coefficients of 11,610 and 33,440 for FeCl3 and o -phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o -phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.
