出版社：American Society for Biochemistry and Molecular Biology
摘要：A new procedure for the preparation of methyl esters from free fatty acids under mild conditions was investigated. Free fatty acids are dissolved in a mixture of chloroform–methanolic HCl–cupric acetate and kept at room temperature for 30 min for complete esterification. The method is suitable for esterification of long-chain acids, such as 18:0, and for very long chain acids, such as 24:0. Fatty acids from brain glycerophosphatides, which included a high concentration of polyenes such as 20:4 (n – 6), 22:4 (n – 6), and 22:6 (n – 3), were also esterified by the same procedure, and neither artifact formation nor loss of unsaturated acids was observed.
