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  • 标题:New colorimetric method for the quantitative estimation of phospholipids without acid digestion
  • 本地全文:下载
  • 作者:R. K. Raheja ; Charanjit Kaur ; Ajit Singh
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1973
  • 卷号:14
  • 期号:6
  • 页码:695-697
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res. , 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1–10 μg of phospholipid phosphorus.
  • 关键词:chromogenic solution ; thin-layer chromatography ; egg yolk phospholipids
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