出版社:American Society for Biochemistry and Molecular Biology
摘要:A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res. , 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1–10 μg of phospholipid phosphorus.