出版社：American Society for Biochemistry and Molecular Biology
摘要：A gas–liquid chromatographic micromethod for quantitation of cholesterol in 20 μl of plasma was developed using 5α-cholestane as an internal standard, saponification with tetramethylammonium hydroxide–isopropanol, and extraction with tetrachloroethylene–methyl butyrate. Cholesterol levels in plasma samples were calculated by comparing cholesterol–cholestane peak height ratios with those of preassayed reference plasma. Over a plasma cholesterol range of 44 to 468 mg/100 ml, the gas–liquid chromatographic micromethod and the automated ferric chloride colorimetric method gave nearly identical results ( r = 0.99) in duplicate aliquots of 131 plasma samples.
