出版社：American Society for Biochemistry and Molecular Biology
摘要：The major lipoprotein density classes (chylomicrons-VLDL, LDL, HDL2 and HDL3) were isolated from human plasma in a two-step ultracentrifugal procedure using the Ti-14 zonal rotor. The isolation of the two major high density lipoprotein subclasses (HDL2 and HDL3) was achieved in a 24-hr run using a nonlinear NaBr gradient in the density range of 1.00-1.40. The lipoproteins with a density 2) without increasing the running time. Furthermore, this procedure proved to be suitable for isolation of plasma lipoproteins from subjects with various types and varying degrees of hyperlipoproteinemia.
关键词：nonlinear density gradient ; continuous linear density gradient ; lipoprotein protein ; lipoprotein lipids
