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  • 标题:Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates.
  • 本地全文:下载
  • 作者:O W Cass ; A E Cowen ; A F Hofmann
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1975
  • 卷号:16
  • 期号:2
  • 页码:159-160
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.
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