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  • 标题:Measurement of apolipoprotein A-I in rat high density lipoprotein and in rat plasma by radioimmunoassay.
  • 本地全文:下载
  • 作者:G Schonfeld ; M S Frick ; A P Bailey
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1976
  • 卷号:17
  • 期号:1
  • 页码:25-29
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A double antibody radioimmunoassay (RIA) for rat apolipoprotein A-I is reported. The ApoA-I isolated from delipidated HDL by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS), and its amino acid composition resembled that reported by others. ApoA-I was iodinated by lactoperoxidase and the resulting 125I-apoA-I was purified by gel filtration. Up to 93% of 125I-apoA-I was precipitable by antibody and greater than 99% of bound 125I-apoA-I was displaced by "cold" apoA-I. Other rat lopoproteins and apolipoproteins did not react in this system. Human plasma were also not reactive, nor were dog, goat, and sheep plasmas.
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