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  • 标题:Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma.
  • 本地全文:下载
  • 作者:J Boberg ; J Augustin ; M L Baginsky
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1977
  • 卷号:18
  • 期号:4
  • 页码:544-547
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.
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