标题：Effects of high-glucose and high-fat diets on concanavalin A binding to rat liver plasma membranes and on the amount and pattern of their glycoprotein carbohydrates.
出版社：American Society for Biochemistry and Molecular Biology
摘要：Purified liver plasma membranes were prepared from rats fed a high-fat, carbohydrate-free diet or a high-glucose, fat-free diet. Membranes from rats fed the high-fat diet bound significantly less 125I-labeled concanavalin A (Con A) than did those from rats fed the fat-free diets. The magnitude of the binding difference increased with increasing concentrations of Con A. Neither association nor dissociation rates of the lectin-receptor complex was affected by diet. The extent of degradation of Con A by liver plasma membrane preparations from rats fed either diet was the same. Chemical analysis of delipidated liver plasma membrane showed that membranes prepared from high-fat diet-adapted rats had significantly lower values for all carbohydrate components measured with the exception of galactose. The results indicate that, in liver cells, a change in plasma membrane glycoproteins is part of the complex adaptation to altered diet composition.
