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  • 标题:Use of a simple enzymatic assay for cholesterol analysis in human bile.
  • 本地全文:下载
  • 作者:H Fromm ; P Amin ; H Klein
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1980
  • 卷号:21
  • 期号:2
  • 页码:259-261
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:An enzymatic technique for cholesterol analysis in serum was applied to human bile. The analytical yield was very satisfactory in experiments in which known amounts of cholesterol were added to untreated, as well as Millipore-filtered, samples of human bile. The analytical results of the enzymatic test agreed closely with those of a method utilizing the Liebermann-Burchard reaction. The enzymatic assay of cholesterol in bile proved to be sensitive and precise. In comparison to other methods of biliary cholesterol determination, it has the advantage of being rapid and simple.
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