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  • 标题:Differential transport of cholesterol and oleic acid in lymph lipoproteins: sex differences in puromycin sensitivity.
  • 本地全文:下载
  • 作者:G V Vahouny ; E M Blendermann ; L L Gallo
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1980
  • 卷号:21
  • 期号:4
  • 页码:415-424
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Adult rats of both sexes were prepared with indwelling drainage catheters in the left thoracic lymphatic duct, and with duodenal infusion catheters. Control and puromycin-treated animals were administered an aqueous test emulsion containing [7alpha-(3)H]cholesterol and [1-(14)C]oleic acid, followed two hours later, by a tracer dose of [1-(14)C]-leucine. Successive 2-hr lymph samples were subjected to ultracentrifugal separations of the major lipoprotein classes. These were specifically extracted for lipids, and for DNA- and lipid-free protein. In both sexes, oleic acid absorption was largely associated with the d
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