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  • 标题:Synthesis of molecular species of glycerophospholipids from diglyceride-labeled brain microsomes.
  • 本地全文:下载
  • 作者:R Roberti ; L Binaglia ; G Porcellati
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1980
  • 卷号:21
  • 期号:4
  • 页码:449-454
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Selectivity of CDP-choline:diacylglycerol choline phosphotransferase and CDP-ethanolamine:diacylglycerol ethanolamine phosphotransferase for molecular species of diglyceride has been studied in rat brain microsomes in vitro. Diglyceride-labeled microsomes were prepared by incubation with labeled sn-glycerol-3-phosphate; the microsomes were then incubated with CDP-choline or CDP-ethanolamine for different time intervals. Experimental data extrapolated to zero-time incubation were taken into account for evaluating species specificity. A small selectivity for diglyceride species has been demonstrated for the choline phosphotransferase, but the ethanolamine phosphotransferase was found to convert hexaenoic diglyceride into phospholipid at the highest rate.
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