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  • 标题:Fractionation of fecal neutral steroids by high performance liquid chromatography.
  • 本地全文:下载
  • 作者:E M Jackson ; C A Kloss ; S T Weintraub
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1985
  • 卷号:26
  • 期号:7
  • 页码:893-897
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5-3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.
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