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  • 标题:A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography.
  • 本地全文:下载
  • 作者:S Bahrami ; H Gasser ; H Redl
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1987
  • 卷号:28
  • 期号:5
  • 页码:596-598
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The advantages of this method are the use of an isocratic solvent system limited to about 30 min and the very good separation of the phosphatidyl-choline fraction from the sphingomyelin fraction. Furthermore, the HPLC method has a better recovery rate than the thin-layer chromatography method, and it can be run under automatic control.
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