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  • 标题:A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
  • 本地全文:下载
  • 作者:J C Khoo ; E Miller ; D I Goldberg
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1987
  • 卷号:28
  • 期号:7
  • 页码:873-877
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.
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