出版社：American Society for Biochemistry and Molecular Biology
摘要：An improved micromethod has been developed for the location of double bonds in some naturally occurring polyenoic acids by oxidative cleavage of olefinic bonds with a mixture of periodate and permanganate (von Rudloff's oxidant) and analysis of the acidic fragments by gas-liquid chromatography. A method is described for calculation of the double-bond rhythm of polyunsaturated fatty acids. The structures of three polyenoic acids, isolated from canine adrenal lipids by methanolysis and preparative gas-liquid chromatography of the methyl esters, were established as 8,11,14-eicosatrienoic acid, 5,8,11,14-eicosatetraenoic acid, and 7,10,13,16-docosatetraenoic acid.
