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  • 标题:A sensitive method for determination of cholesterol growth using model solutions of supersaturated bile.
  • 本地全文:下载
  • 作者:N Busch ; H Tokumo ; R T Holzbach
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1990
  • 卷号:31
  • 期号:10
  • 页码:1903-1909
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:We describe a novel, facile, and highly reproducible spectrophotometric technique to measure a cholesterol crystal growth curve. This method permits a quantitative estimate of the potency or comparative abundance of a given kinetic effector substance. In addition to several internal validation procedures, data are provided that support the usefulness of the technique in the assay of substances with either known promoting activity, i.e., human mucin glycoprotein, or those with known inhibitory activity, i.e., human apolipoprotein A-I.
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