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  • 标题:High-resolution proton nuclear magnetic resonance characterization of seminolipid from bovine spermatozoa.
  • 本地全文:下载
  • 作者:J G Alvarez ; B T Storey ; M L Hemling
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1990
  • 卷号:31
  • 期号:6
  • 页码:1073-1081
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:The high-resolution one- and two-dimensional proton nuclear magnetic resonance (1H-NMR) characterization of seminolipid from bovine spermatozoa is presented. The 1H-NMR data was confirmed by gas-liquid chromatography-mass spectrometric analysis of the partially methylated alditol acetates of the sugar unit, mild alkaline methanolysis of the glyceryl ester, mobility on normal phase and diphasic thin-layer chromatography (HPTLC), and fast atom bombardment mass spectrometry (FAB-MS). The structure of the molecule corresponds to 1-O-hexadecyl-2-O-hexadecanoyl-3-O-beta-D-(3'-sulfo)-galactopyranosyl- sn-glycerol.
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