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  • 标题:Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins.
  • 本地全文:下载
  • 作者:B Y Ishida ; B Paigen
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1992
  • 卷号:33
  • 期号:7
  • 页码:1073-1078
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.
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