出版社：American Society for Biochemistry and Molecular Biology
摘要：Previously we quantified the amounts of immunoreactive apo[a] found in human atherosclerotic lesions extracted sequentially with phosphate-buffered saline (PBS) and guanidine hydrochloride (GuHCl). In this study we have attempted to characterize lipoproteins containing apo[a] in such PBS and GuHCl fractions, obtained from autopsy samples, in order to eventually determine their structure-function relationships critical for evaluating the mechanisms that make them atherogenic. Apo[a] in the PBS extracts migrated slightly ahead of plasma Lp[a] on agarose electrophoresis. Although apo[a] in extracts showed the same isoforms as in plasma in SDS-PAGE, it was also highly fragmented. When a d
